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In situ hybridization of pupal wings using riboprobes

Diane Ramos conducts an in situ hybridization on Bicyclus anynana pupal wings using engrailed riboprobes. Experiment narrated by A. Monteiro.


Here is an in situ larval wing protocol that does not require peripodial membrane removal (pdf).

We use this protocol for our mounting media.


Butterfly macro used for measuring black and white areas in Pieris rapae butterfly wings.Click here to download.