HomeTable of ContentsFeedbackSubscribeHelp/AboutArchivesSearch
Science News Online
Login problems?
Click here.

Science News Books
Science News Books.

Audible Science News
Subscribe to Science News in an audio format.

Science News for Kids
Science News for Kids

Online Features

Math Trek
Mapping Scientific Frontiers

Food for Thought
Formula for Failure

Science Safari
Medieval Science

70 Years Ago in Science News

Free E-mail Alert
Science News e-LETTER.

New Searchable Archive

New PDF Archive

Get Firefox!

Print this article
Email this article to a friend

Week of March 13, 2004; Vol. 165, No. 11 , p. 166

New Green Eyes: First butterfly that's genetically modified

Susan Milius

Scientists have for the first time genetically engineered a butterfly, inserting a jellyfish gene into an African butterfly so that its eyes fluoresce green.


EYE FOR EYES. The natural form of an African tropical butterfly Bicyclus anynana. Inset: The magnified eye of a genetically engineered butterfly glows green.
W. Piel; (inset) Ramos

The butterfly, Bicyclus anynana, serves as an important subject for studies of how genes control development and how those controls evolve, says Antónia Monteiro of State University of New York (SUNY) at Buffalo. She and her colleagues figured out how to use molecular techniques to transform the butterfly and open new research opportunities in genetics, she says. The researchers describe their work in an upcoming issue of the Royal Society of London's Biology Letters.

"I think the transformation is a very important tool," comments Daniel Bopp of the Zoological Institute of the University of Zurich, whose team in 2001 made the first genetically modified housefly. "It's a very targeted way to try to understand the function of a gene," he says.

It's been more than 20 years since researchers first genetically engineered an insect, the laboratory fruit fly. In the past decade, the pace has picked up, and biologists have worked out how to manipulate a wide variety of other insects, including several mosquitoes, screwworms, and two moths—the silkworm and the pink bollworm.

To get genes into a new organism, researchers depend on bits of DNA called transposons, which naturally infiltrate a host's genes. Monteiro, working with Jeffrey M. Marcus of Western Kentucky University in Bowling Green and Diane Ramos of SUNY Buffalo, ferried the jellyfish gene in modified forms of the transposons Hermes, which was originally from a housefly, and piggyBac, from a cabbage looper moth.

The researchers chose the jellyfish gene for a green fluorescent protein that other researchers had used to create glowing green eyes in houseflies and some other insects.

The team injected the gene and a modified transposon into each of more than 10,000 butterfly eggs. About 95 percent of the eggs died. Of the survivors, 5 percent getting piggyBac and 10 percent getting Hermes transposons passed along green-fluorescing eyes to their offspring.

Monteiro plans to use this technique to study genes suspected of creating spots on butterfly wings (SN: 2/15/03, p. 104: Available to subscribers at http://www.sciencenews.org/articles/20030215/bob9.asp). Previous research has shown that similar genes in fruit flies control top-bottom orientation, leg development, and other developmental milestones. Monteiro will, for example, add extra copies of one of these genes and turn it on in unusual places. Ultimately, she says she wants to know, "How is it that the same old genes acquire these totally novel functions in butterfly wings?"

If you have a comment on this article that you would like considered for publication in Science News, send it to editors@sciencenews.org. Please include your name and location.

To subscribe to Science News (print), go to https://www.kable.com/pub/scnw/ subServices.asp.

To sign up for the free weekly e-LETTER from Science News, go to http://www.sciencenews.org/pages/subscribe_form.asp.


Marcus, J.M., D.M. Ramos, and A. Monteiro. In press. Germline transformation of the butterfly Bicyclus anynana. Proceedings of the Royal Society of London B: Biology Letters. Abstract available at http://www.journals.royalsoc.ac.uk/openurl.asp?

Further Readings:

Milius, S. 2003. How the butterfly gets its spots. Science News 163(Feb. 15):104-106. Available to subscribers at http://www.sciencenews.org/articles/20030215/bob9.asp.

For pictures of the butterfly and more on eyespot research, go to http://www.acsu.buffalo.edu/~monteiro/index.html.

For movies of insect development, go to http://www.molbio.wisc.edu/carroll/movies.html.


Daniel Bopp
Zoological Institute
University of Zürich
Winterthurerstrasse 190
CH-8057 Zürich

Jeffrey Marcus
Department of Biology
Western Kentucky University
Bowling Green, KY 42101

Antónia Monteiro
Department of Biological Sciences
State University of New York, Buffalo
Buffalo, NY 14260

Diane Ramos
Department of Biological Sciences
State University of New York, Buffalo
Buffalo, NY 14260

From Science NewsVol. 165, No. 11, March 13, 2004, p. 166.

Subscribe to Science News.
Click OR call


Search WWW
Science News

Sponsored Links
Ticket Offers

Cheap Premium Tickets
The Odd Couple
Philadelphia Eagles

Click here to find resources for enjoying our planet and the universe. Science Mall sells science posters, gifts, teaching tools, and collector items. Finally a store for science enthusiasts, professionals, and kids alike!
Shop at the Science Mall.