In situ hybridization probe preparation

How to use the eppendorf Femtojet 4i to inject Bicyclus anynana embryos

How do design CRISPR guides using a new genome and annotation files:

 If you are working on an organism for which your genome is not available for CRISPR guide design in traditional sites like CRISPR Direct or chopchop. Tirtha Banerjee created a new app that is very helpful. It filters out the best guide for the target region and scans the entire genome in a couple of seconds using GPU acceleration.